- 询价
- Cell Signaling Technology
- 3505
- USA
- 2026年05月29日
- W, IP, IHC-P, IF-IC
- H,M,R,Mk,C,Hr
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- 文献和实验
- 技术资料
- 抗体英文名：
Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb
- 抗原：
synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated
- 应用范围：
W, IP, IHC-P, IF-IC
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,R,Mk,C,Hr
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R Mk (C) (Hr) | Endogenous | 71 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb detects endogenous levels of PABP1 only when methylated on Arg455 or Arg460. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated. Western Blotting
Western blot analysis of extracts from various cell lines using Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb. As expected, PABP1 methylation is decreased in CARM1 knockout (-/-) mouse embryo fibroblasts (MEFs). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Methyl-PABP1 (Arg455/Arg/460) (C60A10) Rabbit mAb in the presence of doubly unmethylated PABP1 control peptide (left) or antigen-specific peptide (right). 
Confocal immunofluorescent analysis of HeLa cells using Methyl-PAPBP1 (Arg 455/Arg460) (C60A10) Rabbit mAb (green). Actin filaments have been labled with DY-554 phalloidin (red). Blue Pseduocolor = DRAQ5™ (fluorescent DNA dye). |
| Background | Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined.
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
Mouse Cytokine ELISPOT Set Catalog No. Capture mAb Detection mAb IFN-γ 88-7384 AN-18 R4-6A2 IL-1α 88-7813 ALF-161 Rabbit
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