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- Cell Signaling Technology
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- 2025年11月24日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Notch3 (8G5) Rat mAb
- 抗原：
fusion protein corresponding to intracellular residues of notch3
- 应用范围：
W
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 适应物种：
H
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 90, 270 | Rat IgG2a |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Notch3 (8G5) Rat mAb detects endogenous levels of total notch3 protein. It recognizes the full-length (270 kDa) and the extracellular truncated fragment containing a short extracellular region, the transmembrane domain and the intracellular region (90 kDa). |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a fusion protein corresponding to intracellular residues of notch3. Western Blotting
Western blot analysis of extracts from HBP-ALL and A204 cells using Notch3 (8G5) Rat mAb. Full-length (FL) and cleaved notch3 proteins are indicated. |
| Background | Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3-4). Notch3 is a member of notch family that is processed in a similar way to notch1 (5). It is expressed primarily in arterial smooth muscle cells (SMC). Mutations altering the number of cysteine residues in the notch3 extracellular region are associated with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), a hereditary angiopathy leading to strokes and dementia in adults (6-8). Recent studies indicate that notch3 is overexpressed in many types of cancers (9-11).
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| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
mAb. It is important to verify that no secondary reagent will bind the BD Fc Block™. Please see description in "The Uses of BD Fc Block™ in Immunophenotyping of Mouse and Rat Leukocytes". For very low-density cell surface markers (e.g.,cytokine
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
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