Applications Key: W=Western Blotting Reactivity Key: H=Human M=Mouse Mk=Monkey Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
LAMP1 (C54H11) Rabbit mAb detects endogenous levels of total LAMP1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Ser140 of human LAMP1.
Western Blotting
Western blot analysis of extracts from HeLa, Neuro2a and COS cells using LAMP1 (C54H11) Antibody.
Background
LAMP1 and LAMP2 (lysosome-associated membrane protein 1 and 2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplam and contains a tyrosine sorting motif which targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. The cellular functions of these two proteins are also overlap - both are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6).