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- Cell Signaling Technology
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
HA-Tag (6E2) Mouse mAb (HRP Conjugate)
- 抗原：
synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA)
- 应用范围：
W
- 供应商：
CST
- 适应物种：
All
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 标记物：
HRP
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W | All | Transfected Only | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | HA-Tag (6E2) Mouse mAb (HRP Conjugate) detects transfected HA-tagged proteins. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) . The antibody was conjugated to HRP under optimal conditions. Western Blotting
Western blot analysis of extracts from COS cells, untransfected or transfected with HA-Akt (lane 2), using HA-Tag (6E2) Mouse mAb (HRP Conjugate). |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
WHOLE MOUNT IN SITU HYBRIDIZATION.Mouse Embryos
expressed in the anterior primitive endoderm of the mouse gastrula" Mechanism of Development, in press - 1997. Modified from the protocol of Harland (1991) and Current Protocols in Molecular Biology. Preparation of the probe.
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