- ￥1700 - 4000
- GeneTex
- 美国
- GTX114949
- 2025年07月14日
- WB, ICC/IF, IHC-P, IHC-Fr
- Rabbit
- Human, Mouse, Rat, Monkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human Occludin. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Monkey
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX114949
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr
- 浓度：
1.25 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Occludin
- 抗体英文名：
Occludin antibody
- 抗体名：
Occludin 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Occludin antibody (GTX114949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Occludin antibody detects Occludin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Occludin stained by Occludin antibody (GTX114949) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Occludin antibody detects Occludin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Occludin protein stained by Occludin antibody (GTX114949) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Occludin(GTX114949) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Occludin antibody detects Occludin protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% PFA at RT for 15 min.
Green: Occludin stained by Occludin antibody (GTX114949) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
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文献和实验Hsiao-Chin Chou et al., J Biomed Sci 2022 (PMID:35705979)
Wu C et al., Free Radic Biol Med 2021 (PMID:34883251)
Watanabe D et al., Pharmaceutics 2021 (PMID:34575559)
Petrella C et al., Pharmacol Res 2021 (PMID:34339837)
Cheng YQ et al., Neural Regen Res 2022 (PMID:34472480)
Zhang LY et al., Evid Based Complement Alternat Med 2020 (PMID:33133218)
Li B et al., Food Funct 2021 (PMID:34002173)
Busatto S et al., J Nanobiotechnology 2020 (PMID:33160390)
Alquisiras-Burgos I et al., Exp Neurol 2020 (PMID:32380020)
Sun L et al., Mol Pharm 2020 (PMID:32011888)
Tian H et al., Adv Healthc Mater 2018 (PMID:29984479)
Jo H et al., Food Chem Toxicol 2017 (PMID:28780155)
He Z et al., Biomaterials 2017 (PMID:28359018)
Hwang D et al., Biomed Pharmacother 2016 (PMID:27876210)
Xuehui Fan et al., Front Pharmacol 2022 (PMID:35685645)
Walter Muranyi et al., iScience 2022 (PMID:35633941)
of interest available; 2) formation of the antibody-antigen complex by adding specific antibody; 3) separation and detection of the immune complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Both polyclonal and monoclonal
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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