- ￥5600
- GeneTex
- 美国
- GTX82787
- 2025年07月13日
- WB, ICC/IF, IHC-P, FACS, ELISA
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human SMAD4 expressed in E. Coli.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX82787
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, ELISA
- 靶点：
SMAD4
- 抗体英文名：
SMAD4 antibody [4G1C6]
- 抗体名：
SMAD4 抗体 [4G1C6]
- 规格：
100 μl
FACS analysis of K562 cells using GTX82787 SMAD4 antibody [4G1C6].
Green : SMAD4
Purple : negative control
WB analysis of A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate using GTX82787 SMAD4 antibody [4G1C6].
IHC-P analysis of lung cancer tissue using GTX82787 SMAD4 antibody [4G1C6].
ICC/IF analysis of NIH3T3 cells using GTX82787 SMAD4 antibody [4G1C6].
Green : SMAD4
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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文献和实验「神药对决癌王」医科院赵玉沛院士/吴晨教授/林东昕院士团队揭示二甲双胍抑制胰腺癌转移新机制
发表了题为 Metformin inhibits pancreatic cancer metastasis caused by SMAD4 deficiency and consequent HNF4G upregulation 的研究成果 (1)。该研究发现二甲双胍可抑制由 SMAD4 缺乏和 HNF4G 上调介导的胰腺癌转移。研究内容SMAD4 缺陷导致 HNF4G 过表达进而促进胰腺癌转移通过对已发表的 8 份 GWAS 数据进行联合分析，作者筛选得到 36 个与胰腺导管腺癌（PDAC）显著相关
case, the antibody should be added in 1:100 to 1:500 final dilution. • For Hsp70, 3A3 and C92 did work, 4G4 and 5A5 did not. 3A3 may be used 1 μl/ sample. • HSE probe must have high-specific activity (>10,000 cpm/fmol) and be double-stranded
. 4. When finished, immerse membrane in Blocking buffer and block overnight. H. Antibodies and detection 1. Incubate with primary antibody diluted in Blocking buffer for 2 hours at room temp. 2. Wash 3 x 10 min with PBST. 1 x10min secondary washing
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