PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
克隆性:
单克隆
适应物种:
Human, Mouse
保质期:
12 month
库存:
50
供应商:
LSM bio
宿主:
Rabbit
应用范围:
ELISA,WB,IHC,IP
浓度:
≥95% as determined by SDS-PAGE
抗体英文名:
anti-SMAD4 antibody,SMAD4 antibody
抗体名:
anti-SMAD4 抗体,SMAD4 抗体
规格:
100μg
SMAD4抗体| SMAD4 antibody
货号 PAab07995
蛋白别名 DPC4 antibody, MADH4
蛋白介绍
In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
产品描述
anti-SMAD4 antibody is a Rabbit Polyclonal antibody againstSMAD4..
建议稀释比例
IHC
Immunohistochemistry of paraffin-embedded human placenta tissue slide using PAab07995( SMAD4 Antibody) at dilution of 1:50
Western blot
HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab07995(SMAD4 antibody) at dilution of 1:600(本抗体仅供体外科研用途,不可用于临床诊断!)