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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human CTCF expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Monkey
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX80397
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA
- 靶点:
CTCF
- 抗体英文名:
CTCF antibody [1D11]
- 抗体名:
CTCF 抗体 [1D11]
- 规格:
100 μl
WB analysis of A31 (1), MCF-7 (2), HeLa (3), HCT116 (4), Jurkat (5), NIH3T3 (6), and Cos7 (7) cell lysate using GTX80397 CTCF antibody [1D11].
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文献和实验Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF
of the PARylation mark. The latter was achieved by ectopic expression of mutated proteins in cells, followed by immunoprecipitation with the polyclonal anti-PAR antibody and Western analysis with a protein-specific antibody. The PARylation-deficient CTCF mutant
:Buffer、体系、孵育时间、spike-in DNA 量以及建库制备和纯化过程。 图 6:不同细胞量 mESCs 的 uliCUT&RUN 数据结果[4] 将不同细胞量 mESC 的 uliCUT&RUN 数据中的 CTCF 和 H3K4me3 测序结果与已有文献发表的 ChIP-seq 数据进行 read 密度可视化比较,发现 10 个细胞起始量的测序结果可以展现出类似 50 万细胞量的结合位点富集热图。其中 CTCF 的结合区更为狭窄集中,而 H3K4me3 则占据相对宽的区段。 ✦
Mapping Networks of Protein‐Mediated Physical Interactions Between Chromatin Elements
England Biolabs) ChIP dilution buffer (see recipe ) Antibody for immunoprecipitation, specific for the protein
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