- ￥5300
- GeneTex
- 美国
- GTX79365
- 2025年07月13日
- WB, ICC/IF, IHC-P, ELISA
- Armenian Hamster
- Human, Mouse, Hamster
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide corresponding to residues N(418) P E R S M N F P N G E G A S S Q R L C(436) of hamster Spi2A protein.
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Hamster
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Hamster
- 目录编号：
GTX79365
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Armenian Hamster
- 应用范围：
WB, ICC/IF, IHC-P, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Serine Protease Inhibitor 2A (Spi2A)
- 抗体英文名：
Serine Protease Inhibitor 2A (Spi2A) antibody [MoFo 29.2]
- 抗体名：
Serine Protease Inhibitor 2A (Spi2A) 抗体 [MoFo 29.2]
- 规格：
100 μg
IHC-P analysis of mouse kidney tissue using GTX79365 Spi2A antibody [MoFo 29.2]. Tissue section was probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC
ICC/IF analysis of Jurkat cells using GTX79365 Spi2A antibody [MoFo 29.2]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC
ICC/IF analysis of BaF-3 cells using GTX79365 Spi2A antibody [MoFo 29.2]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC
WB analysis of NIH3T3 cells using GTX79365 Spi2A antibody [MoFo 29.2].
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of ice-cold PBS containing protease inhibitor cocktail. 5. Prepare 0.2X trypsin diluted in PBS, and add 5 mL of it to the cells. Incubate in 5% CO2 for 5 min at 37°C. 6. Neutralize the trypsin with 10 mL of 10% fetal bovine
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
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