- ￥5300
- GeneTex
- 美国
- GTX79353
- 2025年07月15日
- WB, FACS, ELISA
- Armenian Hamster
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Full length mouse Bcl-3 protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX79353
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Armenian Hamster
- 应用范围：
WB, FACS, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody detects human and mouse Bcl-3 from transfected cells and shows no cross-reactivity to other Bcl isoforms.
- 抗体英文名：
BCL3 antibody [Ham150-3.5]
- 抗体名：
BCL3 抗体 [Ham150-3.5]
- 规格：
100 μg
FACS analysis of BAF-3 cells using GTX79353 BCL3 antibody [Ham150-3.5] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% PFA
FACS analysis of Ramos cells using GTX79353 BCL3 antibody [Ham150-3.5] compared to an isotype control (blue).
Dilution : 1 ug/test for 40 min at room temperature
Fixation : 2% PFA
WB analysis of Bcl-3 transgenic mouse T cells using GTX79353 BCL3 antibody [Ham150-3.5].
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文献和实验mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell pellet, gently resuspend the cells
while vortexing. If Biotinylating, add 150uL of Biotin/Water solution to Antibody while vortexing. 3. Incubate at room temperature for 45minutes. 4. The next step is to separate the coupled from uncoupled FITC or Biotin. This can be done in 2 ways
IMMUNOSCREENING OF WITH ANTIBODY PROBES
in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody
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