- ￥4300
- GeneTex
- 美国
- GTX70020
- 2025年07月06日
- WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX70020
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Clone GT12 binds to linear repetitive epitope in the PG region and allows for selective detection of both native and denatured CA IX without cross-reactivity to other carbonic anhydrases.
- 抗体英文名：
Carbonic Anhydrase IX antibody [GT12]
- 抗体名：
Carbonic Anhydrase IX 抗体 [GT12]
- 规格：
100 μl
Carbonic Anhydrase IX antibody [GT12] detects Carbonic Anhydrase IX protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [GT12] (GTX70020) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
Immunohistochemical analysis of paraffin-embedded renal cell carcinoma (clear cell type) using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.
Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
Flow cytometry on HeLa cells (1x10^6) stained with anti-CAIX antibody [GT12] (GTX70020) at a 1:1000 dilution. HeLa cells were untreated (green) or treated with 200μM CoCl2 (pink) for 48 hr.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200μM CoCl2 for 48hr using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.
Sample (30 μg HeLa whole cell lysate)
A: 24 hr Untreated
B: 24 hr treatment with 100μM CoCl2
C: 24 hr treatment with 200μM CoCl2
D: 48 hr Untreated
E: 48 hr treatment with 100μM CoCl2
F: 48 hr treatment with 200μM CoCl2
Anti-CAIX antibody [GT12] (GTX70020) was used at a dilution of 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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文献和实验Yeh YH et al., J Exp Clin Cancer Res 2018 (PMID:29587825)
Ramchandani D et al., Lab Invest 2016 (PMID:27721473)
Abd-Aziz N et al., Oncol Lett 2015 (PMID:26622817)
Huang WJ et al., PLoS One 2015 (PMID:25738958)
Hou P et al., PLoS One 2014 (PMID:25420025)
Wiegering A et al., Neoplasia 2014 (PMID:25425971)
Pritchett TL et al., Oncogene 2014 (PMID:25023703)
Hwang-Verslues WW et al., Proc Natl Acad Sci U S A 2013 (PMID:23836662)
Tang X et al., Cell Prolif 2021 (PMID:34390064)
Jin X et al., Nat Med 2017 (PMID:29035367)
Davis RM et al., ACS Nano 2018 (PMID:30203645)
Thompson JM et al., Mol Cancer Ther 2018 (PMID:29720560)
【Jounal club】NATURE MEDICINE: 利用蛋白组技术发现糖尿病眼底病变新机制
News and Views: A prize catch for diabetic retinopathy Nature Medicine - 13, 131 - 132 (2007) Article: Extracellular carbonic anhydrase mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation Nature
. Balasubramanian, Artificial chaperoning of insulin, human carbonic anhydrase and hen egg lysozyme using linear dextrin chains-a sweet route to the native state of globular proteins, FEBS Lett. 443 (1999) 215-219. 46. M. Yoshimoto, R. Kuboi, Oxidative refolding
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
Porcine follicle-stimulating hormoneS, 10 mg/L, PHO1[174]Porcine inhibitor of carbonic anhydrase (transferrin family)S, 5 mg/L, α-MF[175]Porcine single-chain interleukin-12S, 4 mg/L,α-MF, active370Porcine leukocyte 12-lipoxygenaseI[176]Porcine lactoferrinS
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