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HIF2 alpha antibody [GT125]

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • GTX632015
  • 2025年07月06日
  • WB, ICC/IF, IP
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.

    • 亚型

      IgG1

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX632015

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Mouse

    • 应用范围

      WB, ICC/IF, IP

    • 浓度

      1 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      HIF2 alpha

    • 抗体英文名

      HIF2 alpha antibody [GT125]

    • 抗体名

      HIF2 alpha 抗体 [GT125]

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
    Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
    Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
    Red: Actin filaments were labeled with Phalloidin.

    产品细节图片2

    HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX632015) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

    产品细节图片3

    HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
    Samples: PC-12 cells mock (left) and treated with 500 μM CoCl2 for 24 hrs (right) were fixed in 4% PFA at RT for 15 min.
    Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
    Blue: Hoechst 33342 staining.

    产品细节图片4

    Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

    产品细节图片5

    HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
    Sample: NIH/3T3 cells were fixed in 4% PFA at RT for 15 min.
    Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
    Blue: Hoechst 33342 staining.

    产品细节图片6

    Immunoprecipitation of HIF2 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF2 alpha antibody [GT125] (GTX632015).
    Western blot analysis was performed using HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:500.
    EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Kundu A et al., J Exp Med 2020 (PMID:32251515)
    Chen L et al., Sci Rep 2016 (PMID:27581364)
    Lin CS et al., Int J Mol Sci 2016 (PMID:27231905)
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    文献支持
    HIF2 alpha antibody [GT125]
    ¥1700 - 4000