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- ￥1700 - 4000
- GeneTex
- 美国
- GTX632015
- 2026年05月25日
- WB, ICC/IF, IP
- Mouse
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX632015
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HIF2 alpha
- 抗体英文名：
HIF2 alpha antibody [GT125]
- 抗体名：
HIF2 alpha 抗体 [GT125]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
Red: Actin filaments were labeled with Phalloidin.
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody (GTX632015) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Samples: PC-12 cells mock (left) and treated with 500 μM CoCl2 for 24 hrs (right) were fixed in 4% PFA at RT for 15 min.
Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
Blue: Hoechst 33342 staining.
Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
HIF2 alpha antibody [GT125] detects HIF2 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% PFA at RT for 15 min.
Green: HIF2 alpha protein stained by HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:100.
Blue: Hoechst 33342 staining.
Immunoprecipitation of HIF2 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF2 alpha antibody [GT125] (GTX632015).
Western blot analysis was performed using HIF2 alpha antibody [GT125] (GTX632015) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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文献和实验Kundu A et al., J Exp Med 2020 (PMID:32251515)
Chen L et al., Sci Rep 2016 (PMID:27581364)
Lin CS et al., Int J Mol Sci 2016 (PMID:27231905)
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
Antibody Screening of Bacteriophage gt11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
Antibody Screening of Bacteriophage gt-11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
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