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NDP52 antibody [GT422]

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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • GTX630396
  • 2025年07月10日
  • WB, ICC/IF, IP
  • Mouse
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

    • 亚型

      IgG1

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX630396

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Mouse

    • 应用范围

      WB, ICC/IF, IP

    • 浓度

      1 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      NDP52

    • 抗体英文名

      NDP52 antibody [GT422]

    • 抗体名

      NDP52 抗体 [GT422]

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
    The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

    产品细节图片2

    NDP52 antibody [GT422] detects NDP52 protein at autophagosome  by immunofluorescent analysis.
    Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
    Green: NDP52 stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
    Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
    Blue: Fluoroshield with DAPI (GTX30920).
    Scale bar= 10 μm.

    产品细节图片3

    NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
    Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec.
    Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
    Blue: Hoechst 33342 staining.
    Scale bar = 10 μm.

    产品细节图片4

    Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.

    产品细节图片5

    NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
    A. 30 μg Jurkat whole cell lysate/extract
    B. 30 μg Raji whole cell lysate/extract
    C. 30 μg NCI-H929 whole cell lysate/extract
    10 % SDS-PAGE
    NDP52 antibody [GT422] (GTX630396) dilution: 1:1000

    产品细节图片6

    NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
    Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% PFA at RT for 15 min.
    Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
    Red: Phalloidin, a F-actin marker.

    产品细节图片7

    NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
    The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.

    产品细节图片8

    NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
    A. 30 μg Huh7 whole cell lysate/extract
    B. 30 μg Hep3B whole cell lysate/extract
    C. 30 μg HepG2 whole cell lysate/extract
    10 % SDS-PAGE
    NDP52 antibody [GT422] (GTX630396) dilution: 1:1000

    产品细节图片9

    Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody [GT422] (GTX630396).
    Western blot analysis was performed using NDP52 antibody [GT422] (GTX630396).
    EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Prabakaran T et al., EMBO J 2018 (PMID:29496741)
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    文献支持
    NDP52 antibody [GT422]
    ¥1700 - 4000