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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX630396
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
NDP52
- 抗体英文名:
NDP52 antibody [GT422]
- 抗体名:
NDP52 抗体 [GT422]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: NDP52 stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.
NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec.
Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT422] (GTX630396) dilution: 1:1000
NDP52 antibody [GT422] detects NDP52 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% PFA at RT for 15 min.
Green: NDP52 protein stained by NDP52 antibody [GT422] (GTX630396) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.
NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX630396) diluted by 1:500.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
NDP52 antibody [GT422] detects NDP52 protein by western blot analysis.
A. 30 μg Huh7 whole cell lysate/extract
B. 30 μg Hep3B whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
NDP52 antibody [GT422] (GTX630396) dilution: 1:1000
Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 μg of NDP52 antibody [GT422] (GTX630396).
Western blot analysis was performed using NDP52 antibody [GT422] (GTX630396).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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文献和实验Prabakaran T et al., EMBO J 2018 (PMID:29496741)
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