- ￥1700 - 4000
- GeneTex
- 美国
- GTX629889
- 2025年07月15日
- WB, ICC/IF
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX629889
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
SQSTM1 / P62
- 抗体英文名：
SQSTM1 / P62 antibody [GT381]
- 抗体名：
SQSTM1 / P62 抗体 [GT381]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hr and 24hr) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629889) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629889) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [GT381] (GTX629889) diluted at 1:500.
SQSTM1 antibody [GT381] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% PFA at RT for 15 min.
Green: SQSTM1 protein stained by SQSTM1 antibody [GT381] (GTX629889) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.
SQSTM1 antibody [GT381] detects SQSTM1 protein by Western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
10 % SDS-PAGE
SQSTM1 antibody [GT381] (GTX629889) dilution: 1:1000
SQSTM1 antibody [GT381] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .
Green: SQSTM1 protein stained by SQSTM1 antibody [GT381] (GTX629889) diluted at 1:500.
Blue: Hoechst 33342 staining.
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
involves conventional 3C methodology: The chromatin is cross-linked, digested with restriction enzymes, and ligated under conditions that favor intramolecular ligation. Immediately after ligation, the chromatin is immunoprecipitated using an antibody
Southern Blotting(Fred Hutchinson Cancer Research Cente
with Blot Wash #1: 2 x 5' at room temp (RT). 3. Wash with Blot Wash #2: 2 x 15' at 55℃. 4. Wash with Buffer 1: briefly (1') at RT. 5. (Optional; see above) Block with Buffer 2: 30' (O/N okay) Save and store at 4℃. 6. Incubate with antibody-conjugate
Phage‐Based Expression Cloning to Identify Interacting Proteins
the interacting protein. This technique leads directly to the isolation of a cDNA encoding the interacting protein, bypassing the need for labor?intensive protein purification, microsequencing, or antibody production.
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