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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The immunogen used to generate this antibody corresponds to 5-Hydroxymethylcytosine / 5-hmC
- 亚型:
IgG2b
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Species independent
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX629701
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
Dot, MeDIP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
5-Hydroxymethylcytosine / 5-hmC
- 抗体英文名:
5-Hydroxymethylcytosine / 5-hmC antibody [GT513]
- 抗体名:
5-Hydroxymethylcytosine / 5-hmC 抗体 [GT513]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
GTX629701 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] (GTX629701) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
GTX629701 5-hydroxymethylcytosine antibody [GT513] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629701) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine
Dotblot analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] with DNA samples
DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] (GTX629701) at a dilution of 1:500.
A: Genomic DNA derived from 293T cells.
B: Genomic DNA derived from human TET-1 transfected 293T cells.
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文献和实验Southern Blotting(Fred Hutchinson Cancer Research Cente
with Blot Wash #1: 2 x 5' at room temp (RT). 3. Wash with Blot Wash #2: 2 x 15' at 55℃. 4. Wash with Buffer 1: briefly (1') at RT. 5. (Optional; see above) Block with Buffer 2: 30' (O/N okay) Save and store at 4℃. 6. Incubate with antibody-conjugate
Phage‐Based Expression Cloning to Identify Interacting Proteins
the interacting protein. This technique leads directly to the isolation of a cDNA encoding the interacting protein, bypassing the need for labor?intensive protein purification, microsequencing, or antibody production.
Bromodeoxyuridine Immunohistochemistry
between the following steps, and use apx. 20 ml for each reagent (step 3-7). 6. 30, 50, 70, 95, 100 x 2, RT, 30 min. each , with slight agitation. This step functions to dehydrate the tissues through an increasing ethanol series: Samples in 70, 95, or 100
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