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5-Hydroxymethylcytosine / 5-hm

C antibody [GT513]
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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • GTX629701
  • 2025年07月13日
  • Dot, MeDIP
  • Mouse
  • Species independent
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      The immunogen used to generate this antibody corresponds to 5-Hydroxymethylcytosine / 5-hmC

    • 亚型

      IgG2b

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Species independent

    • 保质期

      12 months from the shipping date of the product. 

    • 目录编号

      GTX629701

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Mouse

    • 应用范围

      Dot, MeDIP

    • 浓度

      1 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      5-Hydroxymethylcytosine / 5-hmC

    • 抗体英文名

      5-Hydroxymethylcytosine / 5-hmC antibody [GT513]

    • 抗体名

      5-Hydroxymethylcytosine / 5-hmC 抗体 [GT513]

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    GTX629701 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] in MeDIP experiment.
    Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] (GTX629701) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

    GTX629701 5-hydroxymethylcytosine antibody [GT513] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629701) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

    Dotblot analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] with DNA samples
    DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] (GTX629701) at a dilution of 1:500.
    A: Genomic DNA derived from 293T cells.
    B: Genomic DNA derived from human TET-1 transfected 293T cells.

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    图标文献和实验
    相关实验
    • Southern Blotting(Fred Hutchinson Cancer Research Cente

      with Blot Wash #1: 2 x 5' at room temp (RT). 3. Wash with Blot Wash #2: 2 x 15' at 55℃. 4. Wash with Buffer 1: briefly (1') at RT. 5. (Optional; see above) Block with Buffer 2: 30' (O/N okay) Save and store at 4℃. 6. Incubate with antibody-conjugate

    • Phage‐Based Expression Cloning to Identify Interacting Proteins

      the interacting protein. This technique leads directly to the isolation of a cDNA encoding the interacting protein, bypassing the need for labor?intensive protein purification, microsequencing, or antibody production.      

    • Bromodeoxyuridine Immunohistochemistry

      between the following steps, and use apx. 20 ml for each reagent (step 3-7). 6. 30, 50, 70, 95, 100 x 2, RT, 30 min. each , with slight agitation. This step functions to dehydrate the tissues through an increasing ethanol series: Samples in 70, 95, or 100

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