The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC
亚型:
IgG2b
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Species independent
保质期:
12 months from the shipping date of the product.
目录编号:
GTX629765
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
IHC-P, IHC-Fr, Dot, EM, MeDIP
浓度:
0.81 mg/ml (Please refer to the vial label for the specific concentration.)
GTX629765 5-HydroxymethylCytidine antibody [GT13612] in MeDIP experiment. Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.
5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.
GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine
5-HydroxymethylCytidine antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. 5-HydroxymethylCytidine antibody [GT13612] (GTX629765) dilution: 1:100.
Application Reference
Huang R et al., Ann Transl Med 2020 (PMID:32355773)
Wu J et al., J Exp Clin Cancer Res 2019 (PMID:31399111)
Yatagai N et al., Virchows Arch 2019 (PMID:30554333)
Sun J et al., Cell Stem Cell 2018 (PMID:30146412)
Cesar S.Huertas et al., Biosensors and Bioelectronics 2018 120()
Hsieh MC et al., J Pineal Res 2017 (PMID:28718992)
Hsieh MC et al., Sci Rep 2016 (PMID:27857218)