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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX628789
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
0.92 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Histone H2A.XS139ph (phospho Ser139)
- 抗体英文名:
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]
- 抗体名:
Histone H2A.XS139ph (phospho Ser139) 抗体 [GT2311]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 μg HCT116 with CPT 30 μM treatment 24 hr whole cell lysate/extract A. 30 μg HCT116 whole with CPT 30 uM treatment cell lysate/extract B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of histone H2A.X (phospho S139) protein by 2 μg histone H2A.X (phospho S139) antibody (GTX628789) 15% SDS-PAGE The immunoprecipitated histone H2A.X (phospho S139) protein was detected by Human histone H2A.X (phospho S139) antibody (GTX628789) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis.
Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% PFA for 15 min.
Red: H2A.X protein stained by Histone H2A.X (phospho Ser139) antibody (GTX628789) diluted at 1:500.
Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% PFA for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibody (GTX628789) diluted at 1:500. Blue: Hoechst 33342 staining.
Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 μg) were separated by 12%-15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献和实验Lin-Sheng Yu et al., Cancers (Basel) 2021 (PMID:34439214)
Lin BZ et al., Molecules 2020 (PMID:32784458)
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