Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]
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Histone H2A.XS139ph (phospho S

er139) antibody [GT2311]
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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • GTX628789
  • 2025年07月06日
  • WB, ICC/IF, IHC-P, IP
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.

    • 亚型

      IgG1

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX628789

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Mouse

    • 应用范围

      WB, ICC/IF, IHC-P, IP

    • 浓度

      0.92 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      Histone H2A.XS139ph (phospho Ser139)

    • 抗体英文名

      Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]

    • 抗体名

      Histone H2A.XS139ph (phospho Ser139) 抗体 [GT2311]

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    Histone H2A.XS139ph (phospho S

    Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 μg HCT116 with CPT 30 μM treatment 24 hr whole cell lysate/extract A. 30 μg HCT116 whole with CPT 30 uM treatment cell lysate/extract B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of histone H2A.X (phospho S139) protein by 2 μg histone H2A.X (phospho S139) antibody (GTX628789) 15% SDS-PAGE The immunoprecipitated histone H2A.X (phospho S139) protein was detected by Human histone H2A.X (phospho S139) antibody (GTX628789) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

    Histone H2A.XS139ph (phospho S

    Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis.
    Sample: Paraffin-embedded mouse testis.
    Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) dilution: 1:500.

    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

    Histone H2A.XS139ph (phospho S

    Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis.
    Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% PFA for 15 min.
    Red: H2A.X protein stained by Histone H2A.X (phospho Ser139) antibody (GTX628789) diluted at 1:500.
    Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000.
    Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

    Histone H2A.XS139ph (phospho S

    Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

    Histone H2A.XS139ph (phospho S

    Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% PFA for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibody (GTX628789) diluted at 1:500. Blue: Hoechst 33342 staining.

    Histone H2A.XS139ph (phospho S

    Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 μg) were separated by 12%-15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

    Histone H2A.XS139ph (phospho S

    Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Lin-Sheng Yu et al., Cancers (Basel) 2021 (PMID:34439214)
    Lin BZ et al., Molecules 2020 (PMID:32784458)
    Tomita T et al., Nat Commun 2021 (PMID:34135341)
    Chen TY et al., Cell Death Differ 2021 (PMID:33462409)
    Li G et al., J Cell Sci 2021 (PMID:33310909)
    Chakraborty A et al., Nat Commun 2016 (PMID:27703167)
    Honda T et al., Sci Rep 2020 (PMID:31980707)
    Lin ST et al., Front Physiol 2019 (PMID:31681015)
    Wang CY et al., Int J Mol Sci 2019 (PMID:31717306)
    Tamanaha-Nakasone A et al., Sci Rep 2019 (PMID:31015491)
    Chang CY et al., Cell Death Discov 2018 (PMID:30393570)
    Wang Y et al., Neurotox Res 2014 (PMID:23996700)
    En J et al., PLoS Negl Trop Dis 2017 (PMID:28783752)
    Guo J et al., PLoS One 2014 (PMID:24505404)
    Rodriguez A et al., Theor Biol Med Model 2015 (PMID:26385365)
    Zhang Y et al., Cancer Res 2016 (PMID:27262172)
    Ohba S et al., Cancer Res 2014 (PMID:25035396)
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    文献支持
    Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]
    ¥1700 - 4000