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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Immunogen Amino acid sequence - ASWSHPQFEKGA
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX628900
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Strep epitope Tag
- 抗体英文名:
Strep epitope Tag antibody [GT661]
- 抗体名:
Strep epitope Tag 抗体 [GT661]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Strep epitope Tag antibody [GT661] immunoprecipitates strep tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of strep tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of strep tagged protein by 2.5 μg of Strep epitope Tag antibody [GT661] (GTX628900) 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.
Strep epitope Tag antibody [GT661] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% PFA at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT661] (GTX628899) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.
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文献和实验Hori N et al., J Biol Chem 2020 (PMID:32385107)
Aoki E et al., Protein Expr Purif 2017 (PMID:29284141)
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