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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human NEFL expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX60544
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, FACS, ELISA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
NF-L
- 抗体英文名:
NF-L antibody [2G10]
- 抗体名:
NF-L 抗体 [2G10]
- 规格:
100 μg
Sandwich ELISA detection of recombinant full-length NF-L protein using GTX134085 as capture antibody at concentration of 5 μg/mL and GTX60544 as detection antibody was diluted at 1:10000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Sandwich ELISA detection of recombinant full-length NF-L protein using GTX101142 as capture antibody at concentration of 5 μg/mL and GTX60544 as detection antibody was diluted at 1:10000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
ICC/IF analysis of HeLa cells using GTX60544 NF-L antibody [2G10].
Green : NF-L
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
IHC-P analysis of lung cancer tissue using GTX60544 NF-L antibody [2G10].
WB analysis of HeLa (1) and Jurkat (2) cell lysate using GTX60544 NF-L antibody [2G10].
ELISA analysis of antigen using GTX60544 NF-L antibody [2G10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
FACS analysis of Jurkat cells using GTX60544 NF-L antibody [2G10].
Green : NF-L
Red : negative control
IHC-P analysis of human brain tissue using GTX60544 NF-L antibody [2G10].
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文献和实验Immunohistochemistry / Immunocytochemistry Procedures
In these techniques an antibody is used to link a cellular antigen specifically to a stain that can be more readily seen with a microscope. Detection of antigens in tissues is known as
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