- ￥1700 - 4000
- GeneTex
- 美国
- GTX101142
- 2025年07月14日
- WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human NF-L. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101142
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
NF-L
- 抗体英文名：
NF-L antibody
- 抗体名：
NF-L 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
NF-L antibody detects NF-L protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% PFA at RT for 15 min.
Green: NF-L protein stained by NF-L antibody (GTX101142) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
NF-L antibody detects NF-L protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: NF-L protein stained by NF-L antibody (GTX101142) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Sandwich ELISA detection of recombinant full-length NF-L protein using GTX101142 as capture antibody at concentration of 5 μg/mL and GTX60544 as detection antibody was diluted at 1:10000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using 68kDa Neurofilament Light(GTX101142) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Mouse tissue extract (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-L antibody (GTX101142) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sandwich ELISA detection of recombinant full-length NF-L protein using GTX101142 as capture antibody at concentration of 5 μg/mL and GTX24572 as detection antibody was diluted at 1:1000. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.
Human brain (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NF-L antibody (GTX101142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 ug of whole cell lysate)
A: PC-3
B: U87-MG
C: SK-N-SH
7.5% SDS PAGE
GTX101142 diluted at 1:10000
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