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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
RFXAP (Regulatory factor X-associated protein), using the recombinant protein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX60368
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
ChIP assay
- 浓度:
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点:
RFXAP
- 抗体英文名:
RFXAP antibody - ChIP grade
- 抗体名:
RFXAP 抗体 - ChIP grade
- 规格:
50 μg
ChIP analysis of sheared chromatin from 2x10⁶ NALM cells using GTX60368 RFXAP antibody - ChIP grade. An anti-flag antibody (lane 2) was used as negative IP control. This figure shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DoB, used a negative PCR control (upper panel).
Antibody amount : 5-7μg
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文献和实验Herring Sperm DNA37% Formaldehyde (ACS reagent grade)1.25 M glycineProtocol: (Generalized for all cell types- inquire for details regarding particular cell types) For all the following steps,use the pipets that are specifically designated for ChIP use
Characterization and Quality Control of Antibodies Used in ChIP Assays
, modifying enzymes, and chromatin-interacting proteins. The final purpose of the characterization and QC is to label antibodies as chromatin immunoprecipitation (ChIP) grade. Indeed, the ChIP method is extensively used in epigenetics to study gene regulation
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Abstract Table of Contents Materials Figures Literature Cited Abstract ChIP?chip combines
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