- ￥5000
- GeneTex
- 美国
- GTX60364
- 2025年07月15日
- WB, ICC/IF, ChIP assay
- Mouse
- Human, Zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Histone H4 containing the trimethylated lysine 20 (H4K20me3), using a KLH-conjugated synthetic peptide.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Zebrafish
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60364
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, ChIP assay
- 靶点：
Histone H4K20me3 (trimethyl Lys20)
- 抗体英文名：
Histone H4K20me3 (trimethyl Lys20) antibody [GT9009]
- 抗体名：
Histone H4K20me3 (trimethyl Lys20) 抗体 [GT9009]
- 规格：
50 μl
ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60364 Histone H4K20me3 (trimethyl Lys20) antibody [GT9009]. Two different quantities of antibody (3 and 12 μl per ChIP experiment) were analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive gene TSH2B. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ICC/IF analysis of 4% PFA fixed HEK293T cells using GTX60364 Histone H4K20me3 (trimethyl Lys20) antibody [GT9009].
Green : Primary antibody
Pink : CREST antibody
Blue : DAPI
Dilution : 1:200
WB analysis of histone extracts (15 μg) from HeLa cells using GTX60364 Histone H4K20me3 (trimethyl Lys20) antibody [GT9009].
Dilution : 1:50
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文献和实验IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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