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- 详细信息
- 技术资料
- 免疫原:
Histone H4 containing the trimethylated lysine 20 (H4K20me3), using a KLH-conjugated synthetic peptide.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Zebrafish
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX60364
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, ChIP assay
- 靶点:
Histone H4K20me3 (trimethyl Lys20)
- 抗体英文名:
Histone H4K20me3 (trimethyl Lys20) antibody [GT9009]
- 抗体名:
Histone H4K20me3 (trimethyl Lys20) 抗体 [GT9009]
- 规格:
50 μl
ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60364 Histone H4K20me3 (trimethyl Lys20) antibody [GT9009]. Two different quantities of antibody (3 and 12 μl per ChIP experiment) were analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the inactive gene TSH2B. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ICC/IF analysis of 4% PFA fixed HEK293T cells using GTX60364 Histone H4K20me3 (trimethyl Lys20) antibody [GT9009].
Green : Primary antibody
Pink : CREST antibody
Blue : DAPI
Dilution : 1:200
WB analysis of histone extracts (15 μg) from HeLa cells using GTX60364 Histone H4K20me3 (trimethyl Lys20) antibody [GT9009].
Dilution : 1:50
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