- ￥1700 - 4000
- GeneTex
- 美国
- GTX128960
- 2025年07月14日
- WB, ICC/IF, IHC-P, Dot
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys20 of human Histone H4. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX128960
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, Dot
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H4K20me3 (trimethyl Lys20)
- 抗体英文名：
Histone H4K20me3 (trimethyl Lys20) antibody
- 抗体名：
Histone H4K20me3 (trimethyl Lys20) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide
Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody (GTX128960) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.
Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
15 % SDS-PAGE
Histone H4 (tri methyl Lys20) antibody (GTX128960) dilution: 1:2000
Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验-Histone H3 和 MBD1 条带相同，简单调了下对比度；Ac-Histone H4 和 HDAC1 条带相同，180 度旋转即可。（同一个图中出现相同条带，能不能长点儿心，别把读者当傻子。。。）图片来源于论文第二篇文章于 2011 年发表，2018 年遭撤稿，目前已经被引用 118 次。图片来源于 pubmed这篇文章撤稿的主要原因同样是 WB 条带重复利用，不过这次作者聪明多了，在不同的图中出现相同条带，甚至将之前发表过的图重新利用。眼尖的小伙伴看出来了吗，3E 和 5B 的β-actin 相同
组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白；在细胞正常 pH 值时，组蛋白带有正电荷，这样它们就可以和 DNA 结合，这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实，组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg ， Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白： H1 ， H2A ， H2B ， H3
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
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