Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Venezuelan equine encephalitis virus
保质期:
12 months from the shipping date of the product.
抗原来源:
Venezuelan equine encephalitis virus
目录编号:
GTX636976
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IHC-P (cell pellet)
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
This antibody was raised against Venezuelan Equine Encephalitis Virus nsP3, and it does not cross-react with Saint Louis encephalitis virus, La Crosse virus, Western Equine Encephalitis Virus, and Eastern Equine Encephalitis Virus.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Venezuelan equine encephalitis virus nsp3 antibody [HL1502] detects Venezuelan equine encephalitis virus nsp3 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% PFA at RT for 15 min.Green: Venezuelan equine encephalitis virus nsp3 stained by Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Venezuelan equine encephalitis virus nsp3 antibody [HL1502] detects Venezuelan equine encephalitis virus nsp3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mock and VEEV nsp3 (Venezuelan equine encephalitis virus) - transfected 293T cell pellet.Green: Venezuelan equine encephalitis virus nsp3 stained by Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunofluorescent analysis of Venezuelan equine encephalitis virus infected cells using Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976).Sample: Multiple virus infected cells slide.Green: Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.