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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of threonine 315 (E-W-T(p)-D-D) derived from human Cyclin H.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX55316
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Cyclin H (phospho Thr315)
- 抗体英文名:
Cyclin H (phospho Thr315) antibody
- 抗体名:
Cyclin H (phospho Thr315) 抗体
- 规格:
100 μl
WB analysis of extracts from JK cells (lane 2) using GTX55316 Cyclin H (phospho Thr315) antibody.
Left : Primary antibody pre-incubated with the antigen specific peptide
Right : Primary antibody
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文献和实验Sch?tz M et al., Viruses 2021 (PMID:34198986)
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors
the DNA in a population of cells with propidium iodide and then determining the cell-cycle profile of that population by fluorescence-activated cell sorter (FACS) or by measuring 3 H-thymidine incorporation into DNA as it is being replicated.
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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