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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of threonine 315 (E-W-T(p)-D-D) derived from Human Cyclin H .
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Polyclonal
- 适应物种:
Human;Mouse
- 保质期:
12 months
- 抗原来源:
Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB
- 靶点:
Uniprot:P51946
- 抗体英文名:
Cyclin H (Phospho-Thr315) Antibody
- 规格:
50ul/100ul
配方:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
应用详情:
Western blotting: 1:500~1:1000
图片:

Western blot analysis of extracts from JK cells (Lane 2), using Cyclin H (Phospho-Thr315) Antibody #11689. The lane on the left is treated with antigen-specific peptide.
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文献和实验CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors
the DNA in a population of cells with propidium iodide and then determining the cell-cycle profile of that population by fluorescence-activated cell sorter (FACS) or by measuring 3 H-thymidine incorporation into DNA as it is being replicated.
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
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