- ￥5300
- GeneTex
- 美国
- GTX54111
- 2025年07月11日
- WB, ICC/IF, IHC-P, Dot, ChIP assay
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic methylated peptide corresponding to residues surrounding K79 of human histone H3
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX54111
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, Dot, ChIP assay
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3K79me3 (tri-methyl Lys79)
- 抗体英文名：
Histone H3K79me3 (tri-methyl Lys79) antibody
- 抗体名：
Histone H3K79me3 (tri-methyl Lys79) 抗体
- 规格：
100 μl
Dot blot analysis of all sorts of methylation peptides using GTX54111 Histone H3K79me3 (tri-methyl Lys79) antibody.
Dilution : 1:1000
ChIP analysis of 293 cell lysate using GTX54111 Histone H3K79me3 (tri-methyl Lys79) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
WB analysis of HeLa cell lysate using GTX54111 Histone H3K79me3 (tri-methyl Lys79) antibody.
Dilution : 1:1000
Loading : 25μg per lane
IHC-P analysis of rat testis tissue using GTX54111 Histone H3K79me3 (tri-methyl Lys79) antibody.
Dilution : 1:200
ICC/IF analysis of 293T cells using GTX54111 Histone H3K79me3 (tri-methyl Lys79) antibody.
Blue : DAPI
IHC-P analysis of mouse testis tissue using GTX54111 Histone H3K79me3 (tri-methyl Lys79) antibody.
Dilution : 1:200
IHC-P analysis of human mammary cancer tissue using GTX54111 Histone H3K79me3 (tri-methyl Lys79) antibody.
Dilution : 1:200
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文献和实验Methyl DNA Immunoprecipitation
can be done. This methylation analysis is highly specific due to the use of a well-characterized monoclonal antibody and each IP is directly quality controlled: two essential keys for reliable results. In addition, the kit protocol is fast and user-friendly. The METHYL kit
组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白；在细胞正常 pH 值时，组蛋白带有正电荷，这样它们就可以和 DNA 结合，这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实，组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg ， Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白： H1 ， H2A ， H2B ， H3
Histone lysine and arginine methylation involved in gene activation and silencing is dynamically regulated. However, partly limited to the research technologies previously available, the dynamics of global histone methylation on a site
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