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- 文献和实验
- 技术资料
- 免疫原:
A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX54109
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, Dot, ChIP assay
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Histone H3K36me3 (tri-methyl Lys36)
- 抗体英文名:
Histone H3K36me3 (tri-methyl Lys36) antibody
- 抗体名:
Histone H3K36me3 (tri-methyl Lys36) 抗体
- 规格:
100 μl
WB analysis of various sample lysates using GTX54109 Histone H3K36me3 (tri-methyl Lys36) antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane
ICC/IF analysis of 293T cells using GTX54109 Histone H3K36me3 (tri-methyl Lys36) antibody.
Blue : DAPI
Dot blot analysis of all sorts of methylation peptides using GTX54109 Histone H3K36me3 (tri-methyl Lys36) antibody.
IHC-P analysis of mouse brain tissue using GTX54109 Histone H3K36me3 (tri-methyl Lys36) antibody.
Dilution : 1:100
IHC-P analysis of human breast cancer tissue using GTX54109 Histone H3K36me3 (tri-methyl Lys36) antibody.
Dilution : 1:100
ChIP analysis of 293T cell lysate using GTX54109 Histone H3K36me3 (tri-methyl Lys36) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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文献和实验9.19 Nature:揭示组蛋白标记 H3K36me2 招募 DNMT3A 并影响基因间 DNA 甲基化
中心、洛克菲勒大学和加拿大麦吉尔大学等研究机构的研究人员报道 NSD1 介导的 H3K36me2 是在基因间区域招募 DNMT3A 和维持 DNA 甲基化所必需的。这项新的研究揭示了一种跨染色质调节途径,这种调节突进将异常的基因间 CpG 甲基化与人类肿瘤生长和过度发育相关联在一起。原文检索:The histone mark H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape ② Nature 突破
Identifying Differential Histone Modification Sites from ChIPseq Data
cell (NPC). We demonstrated that the H3K27me3 DHMSs identified by our approach are of high sensitivity, specificity, and technical reproducibility. ChIPDiff was further applied to uncover the differential H3K4me3 and H3K36me3 sites between different
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
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