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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-430 of human CHRM5 (NP_036257.1).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX53955
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
mAChR M5
- 抗体英文名:
mAChR M5 antibody
- 抗体名:
mAChR M5 抗体
- 规格:
100 μl
WB analysis of various sample lysates using GTX53955 mAChR M5 antibody.
Dilution : 1:1000
Loading : 25μg per lane
IHC-P analysis of rat brain tissue using GTX53955 mAChR M5 antibody.
Dilution : 1:100
ICC/IF analysis of U2OS cells using GTX53955 mAChR M5 antibody.
Blue : DAPI
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文献和实验在做对小鼠成纤维细胞的整个基因组的甲基化水平检测,我是想要关于免疫化学法中提及的关于anti-m5C的一抗和荧光标记的二抗的选择。我不知道怎么选择抗体。希望有这方面经验的专家或者有文献的前辈给我发一下吧。非常感谢。很急!!!!还要谢谢前面那位前辈。非常感谢。 藤崽 你说的是全基因组甲基化抗体吧,就是抗5-methyl cytosine的抗体,很多公司都有的,你查一下,我们用的是abcam公司的,四千左右,二抗就是普通的国产就行了
Important in phagocytosis via receptor-mediated endocytosis of IgG-antigen complexes Mediates antigen capture for presentation to T cells, antibody-dependent cellular cytotoxicity, release of cytokines and reactive oxygen intermediates Positive
Wash Buffer)25 ml 1mliCl5 ml 10% IGEPAL5 ml 10% Deoxycholate1 ml 1M Tris-8.1200μl 0.5M EDTA64 ml WaterProtease inhibitors (add 10μl of each to 10 ml of PBS or sonication buffer)Leupeptin 2 mg/ml in waterAprotinin 2 mg/ml in waterPMSF 0.2 M5 M NaCl1X TE
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