- ￥5300
- GeneTex
- 美国
- GTX50278
- 2025年08月21日
- WB, IHC-P
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of threonine 183 (M-M-T(p)-P-Y) derived from human JNK.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX50278
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody is raised against JNK1/2 phosphorylated at Thr183. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221.
- 抗体英文名：
JNK (phospho Thr183) antibody
- 抗体名：
JNK (phospho Thr183) 抗体
- 规格：
100 μl
IHC-P analysis of human breast carcinoma tissue using GTX50278 JNK (phospho Thr183) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
WB analysis of extracts from 293 cells treated with UV or calf intestinal phosphatase (CIP) using GTX50278 JNK (phospho Thr183) antibody.
WB analysis of extracts from 293 cells using GTX50278 JNK (phospho Thr183) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide
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文献和实验greater selectivity for JNK as compared to ERK1 and p38-2 MAP kinases. Inhibits the phosphorylation of c-Jun and blocks cellular expression of IL-2, IFN-γ, TNF-α, and COX-2. Blocks IL-1-induced accumulation of phospho-Jun and induction of c-Jun
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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