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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX48825
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IP, ELISA, IHC
- 浓度:
1.3 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody reacts with keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa) and keratin 19 (40 kDa) derived from humans.
- 抗体英文名:
pan Cytokeratin antibody [C-11]
- 抗体名:
pan Cytokeratin 抗体 [C-11]
- 规格:
50 μg
Immunofluorescence Microscopy of GeneTex Anti-Keratin antibody (GTX48825) was used with GeneTex Dylight 488 goat anti-mouse (shown in green) to detect Keratin by Immunofluorescence. In the same experiment, GeneTex polyclonal Anti-HDAC-1 antibody was used with Anti-Rabbit IgG (shown in red) to detect HDAC-1.
Western blot using GeneTex Immunochemical's Mouse Mab-anti-Keratin antibody. This antibody recognizes a single 56 kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximatley 100 μg of keratin from human epidermis was applied under reducing conditions. 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat anti-Mouse IgG [H&L] and visualization using the OdysseyR Infrared Imaging System developed by LI-COR.
Immunofluorescence microscopy using GeneTex Immunochemical's Mouse Mab-anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 um where the image is taken near the bottom of the cell. Use FITC a 1:2,000 dilution of FITC-conjugated Goat anti-Mouse IgG [H&L] for detection.
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文献和实验Ordonez NG et al., Hum Pathol 2013 (PMID:23427873)
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