- ￥4700
- GeneTex
- 美国
- GTX48820
- 2025年07月30日
- WB, ICC/IF, ELISA
- Rabbit
- Yeast
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- 详细信息
- 技术资料
- 免疫原：
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Yeast
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Yeast
- 目录编号：
GTX48820
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, ELISA
- 浓度：
5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Ulp1
- 抗体英文名：
Ulp1 antibody
- 抗体名：
Ulp1 抗体
- 规格：
100 μg
Western Blot of Rabbit Anti-ULP1 Antibody. (in duplicate) Lane 1: 50ng ULP1CD. Lane 2: 25ng ULP1CD. Lane 3: 12.5ng ULP1CD. Lane 4: 6.25ng ULP1CD. Lane 5: 3.13ng ULP1CD. Primary Antibody: Anti-ULP1 at 1:1,000 dilution O/N at 4ºC. Secondary Antibody: Goat Anti-Rabbit HRP 1:5,000 RT for 30 min.
Western blot using GeneTex's Affinity Purified anti-Yeast ULP-1 antibody shows detection of a truncated ULP-1 fusion protein (arrowhead). Increasing concentrations of yeast ULP-1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TBST, and probed overnight at 4ºC with a 1:1000 dilution of anti-yULP-1 antibody in 5% non-fat dry milk in TBST. Detection occurred using a 1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
Western blot using GeneTex's Affinity Purified anti-Yeast ULP-1 antibody was used to confirm the specificity of the antibody. SDS-PAGE of 2 μg of ULP-1 homologues from other sources (lanes 2 through 9). After blocking for 1 hour with 5% non-fat dry milk in TBST, the blot was probed overnight at 4ºC with a 1:1,000 dilution of anti-yULP1 antibody detected as above. This antibody is specific for yeast ULP1 and does not react with ULP1 from related sources including human SENP.
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