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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
MBP-fusion protein corresponding to Borrelia burgdorferi Surface Lipoprotein p27 protein.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Borrelia burgdorferi
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Borrelia burgdorferi
- 目录编号:
GTX48799
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Surface Lipoprotein P27
- 抗体英文名:
Surface Lipoprotein P27 antibody
- 抗体名:
Surface Lipoprotein P27 抗体
- 规格:
50 μg
Western blot showing detection of 0.1 μg of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4ºC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
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文献和实验Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
Lipoprotein Isolation and Analysis
Lipoprotein Isolation and Analysis Since lipids are not readily soluble in aqueous media, they are normally transported together with protein components. The resulting lipoproteins are composed of the lipid components (mostly buried
Lipoprotein lipase (LPL) is an N -inked glycoprotein of approximately 57 kDa that is synthesized by a numbel of tissues, including white and brown adipose tissue, heart, skeletal muscle, neonatal liver and brain (1 ,2 ). In these tissues
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