- ￥5300
- GeneTex
- 美国
- GTX48730
- 2025年09月03日
- WB, IP, ELISA
- Rabbit
- Saccharomyces cerevisiae
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Saccharomyces cerevisiae
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Saccharomyces cerevisiae
- 目录编号：
GTX48730
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IP, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Mer2 (phospho Ser30)
- 抗体英文名：
Mer2 (phospho Ser30) antibody
- 抗体名：
Mer2 (phospho Ser30) 抗体
- 规格：
50 μg
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phos-phorylated Mer2 but not phosphatase treated or mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody appears to be specific for phosphorylated Mer2 at the S30 position with negligible cross reactivity against unphosphorylated protein. The primary antibody was used at a 1:5,000 dilution.
Western blot using GeneTex's affinity purified anti-S.cerevisiae Mer2 pS30 antibody shows detection of phosphorylated Mer2 in whole cell extracts. Cells were either wild type (+) or contained mer2 deletions (D). Extracts were prepared from cells 4hr after initiation of meiosis. Proteins were obtained using TCA precipitation. The primary antibody was used at a 1:7,500 dilution. Secondary antibody was used at 1:5,000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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