- ￥5300
- GeneTex
- 美国
- GTX36254
- 2025年07月16日
- WB, ICC/IF, IHC-P, IHC-Fr, PLA
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
CaMKII synthetic peptide with oxidized Met281/282
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX36254
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, PLA
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Mouse. Predicted to react with human based on 100% sequence identity.
- 抗体英文名：
CaMKII (oxidized) antibody
- 抗体名：
CaMKII (oxidized) 抗体
- 规格：
50 μl
Immunofluorescent staining of heart sections from mice treated with saline, AngII, or Isoproterenol and probed for oxidized or total CaMKII. Red staining is positive for oxidized or total CaMKII, and blue staining is positive for nuclei. Mice lacking a critical subunit of NADPH oxidase (p47-/-) did not show increased levels of oxidized CaMKII in response to AngII.
Immunofluorescent staining of heart sections from WT and MsrA-/- mice (a model of heart disease) treated with AngII and probed for oxidized (GTX36254) or total CaMKII and blue staining for nuclei.
Immunoblot of oxidized WT CaMKII probed with antiserum against oxidized M281/282 with increasing ratios of oxidized peptide.
Immunoblot of WT CaMKII and M281/282V mutant after no treatment, oxidation, or autophosphorylation probed with antibodies against total, autophosphorylated, or oxidized M281/282 CaMKII (GTX36254).
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文献和实验Yang X et al., Redox Biol 2020 (PMID:31986467)
Chen HH et al., J Ethnopharmacol 2019 (PMID:31626909)
Shi T et al., Neurobiol Learn Mem 2019 (PMID:31672630)
Chen WJ et al., J Mol Cell Cardiol 2019 (PMID:31419440)
McCluskey C et al., Vascul Pharmacol 2019 (PMID:31051256)
Miller BA et al., J Cell Physiol 2019 (PMID:30637731)
Jiang H et al., Life Sci 2019 (PMID:30472297)
Montnach J et al., J Mol Cell Cardiol 2018 (PMID:30144447)
Martin TP et al., Cell Signal 2018 (PMID:30059730)
Dewenter M et al., Circ Heart Fail 2017 (PMID:28487342)
Maity B et al., Cancer Res 2017 (PMID:29141899)
Zuo S et al., Europace 2017 (PMID:29045723)
Ingoglia G et al., Free Radic Biol Med 2017 (PMID:28400318)
An M et al., Anat Cell Biol 2015 (PMID:26770873)
Yeh YH et al., Basic Res Cardiol 2016 (PMID:27562817)
Popescu I et al., Cardiovasc Res 2016 (PMID:27131508)
Erickson JR et al., Cell 2008 (PMID:18455987)
Zhao J et al., Redox Biol 2023 (PMID:36603528)
Hu Q et al., Alcohol Clin Exp Res 2022 (PMID:35315077)
Calum J McMullen et al., Front Cardiovasc Med 2020 (PMID:33598481)
Julian Mustroph et al., Front Immunol 2021 (PMID:34745111)
Xian Luo et al., Oxid Med Cell Longev 2022 (PMID:35387264)
Joseph LC et al., Sci Rep 2021 (PMID:34497331)
Jiang H et al., Oxid Med Cell Longev 2021 (PMID:34527176)
Woo M et al., Transl Clin Pharmacol 2021 (PMID:34235122)
Pradhan K et al., J Immunol 2021 (PMID:34031144)
Jiang H et al., Biomed Res Int 2020 (PMID:33102583)
Post-electrophoretic identification of oxidized proteins
for the staining process are (a) electrophoresis, (b) electroblotting onto PVDF membranes, (c) derivatization of carbonyls with 2,4-DNP, (d) immunostaining with anti DNP antibody, and (e) protein staining with colloidal gold.
8-Oxoguanine (8-oxoG), an oxidized form of guanine, is one of the major mutagenic lesions generated under oxidative stress. Oxidative damage in mitochondrial DNA has been implicated as a causative factor for a wide variety of degenerative
Conjugation of Antibodies to Horseradish Peroxidase
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups
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