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- ￥6800
- GeneTex
- 美国
- GTX33606
- 2026年05月27日
- ICC/IF, FACS, Dot, ELISA, IHC, MeDIP
- Rabbit
- Species independent
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- 文献和实验
- 技术资料
- 免疫原：
BSA-conjugated 5-methylcytosine.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Species independent
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX33606
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
ICC/IF, FACS, Dot, ELISA, IHC, MeDIP
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.
- 抗体英文名：
5-Methylcytosine / 5-mC antibody [RM231]
- 抗体名：
5-Methylcytosine / 5-mC 抗体 [RM231]
- 规格：
50 μg
ICC/IF analysis of HeLa cells using GTX33606 5-Methylcytosine / 5-mC antibody [RM231].
Red : Primary antibody
Green : Actin
Blue : DAPI
ELISA analysis of HeLa cell genomic DNA using GTX33606 5-Methylcytosine / 5-mC antibody [RM231].
Dilution : 1 ug/mL or 3 ug/mL of RM231 was used as the primary antibody.
MeDIP analysis of 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) spiked in 1ug of genomic DNA isolated from HeLa cells using GTX33606 5-Methylcytosine / 5-mC antibody [RM231]. MeDIP was performed at a 10:1 DNA:Ab ratio. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
ELISA analysis of unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA using serial diluted GTX33606 5-Methylcytosine / 5-mC antibody [RM231].
FACS analysis of HEK293 cells using GTX33606 5-Methylcytosine / 5-mC antibody [RM231]. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with GTX33606 (Blue) or with a negative control PEG antibody (Red).
Dot blot analysis of 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA using GTX33606 5-Methylcytosine / 5-mC antibody [RM231].
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文献和实验Naoshi Kojima et al., Analytica Chimica Acta 2018
Kojima N et al., Biosens Bioelectron 2020 (PMID:32763827)
A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples
to cytosine-6-sulfonate, deamination to uracil-6-sulfonate, and desulfonation to uracil. However, since 5-methylcytosine residues are nonreactive, they remain intact. The bisulfite-converted DNA is then amplified with specific primers designed for converted
S ci. 4 , 803-807. 20. L i a n g , , W u , F. S . , W a n g , D. W , , S u n , D. F. a n d J i a , X . ( 2005 ) W h ea t tra n sfo rm a tio n b y (declropora tio n w ith rin g electro d e. Yi Chuan Xue Bao 32, 66-71
EXAMINING THE FUNCTION OF PROTEINS AND PROTEIN NETWORKS WITH THE YEAST TWO-HYBRID SYSTEM
2 at a sufficiently low level to be used for an interactor hunt. However, if one were to then attempt to thoroughly screen a library of 3 x 106 individual cDNAs by plating over 3 x 106 CFU onto the leu- selection plates, at least 3000 colonies would form
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