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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A phospho specific peptide corresponding to residues surrounding S189 of human ERK3
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX32415
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
ERK3 (phospho Ser189)
- 抗体英文名:
ERK3 (phospho Ser189) antibody
- 抗体名:
ERK3 (phospho Ser189) 抗体
- 规格:
100 μl
WB analysis of 293 cells treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight using GTX32415 ERK3 (phospho Ser189) antibody.
Dilution : 1:1000
Loading : 25μg per lane
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文献和实验Determination of ERK Activity: Anti-phospho-ERK Antibodies and In Vitro Phosphorylation
The ERK signaling cascade is composed of several protein kinases that sequentially activate each other by phosphorylation. This pathway is a central component of a complex signaling network that regulates important cellular processes
memoday 最近在做一个信号通路的下游磷酸化水平检测,但是做western没有看到任何条带,包括p-ERK,p85。麻烦各位战友帮我分析一下可能原因吧: 1.293T细胞转染质粒(带外源基因),24h 2.无血清培养18h 3.配体(外源基因是该配体的膜受体)激活,37度,10min 4.PLB(promega公司)裂解细胞,做western,抗体是SANTA CRUZ公司的p-ERK (Tyr 204),以及cell
liuxing0626 各位学长好: 我买的ProteinTECH公司的 ERK1 抗体 western blot 条带有两条,公司的说明书也是两条,请问这是个怎么情况?而且我要做ERK1/2。但是ERK2也是单买的,混起来用的话会不会出来3条带了。 ERK1产品地址http://www.ptglab.com/Products/ERK1-Antibody-11257-1-AP.htm liuxing0626
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