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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide: AEDDPYLGRFEQMF conjugated to KLH.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX27549
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ELISA, EMSA
- 浓度:
80 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
NFkB p105/p50
- 抗体英文名:
NFkB p105/p50 antibody
- 抗体名:
NFkB p105/p50 抗体
- 规格:
100 μl
Western blot of HeLa cell extract. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
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文献和实验Adrianto I et al., Nat Genet 2011 (PMID:21336280)
renamed rel A) and p50 (recently renamed NFKB1) ( 3 , 4 ). Both of these sub-units belong to the rel famtly of transcription factors with homology in the amino terminus ( 5 , 6 ). Since the original identification of these factors
激活-核转运检测试剂盒仅染色p65,不染色RelB、C-Rel、p50和p52。使用本试剂盒染色后NF-κB呈红色荧光,细胞核呈蓝色荧光.思考信号转导和检测的理论 我做了这样的回答(个人意见.大家可以讨论!): 这种理论上来讲可行,但是实际操作可能拿不到你想要得结果,原因很简单,凭染色来判断是很难区分程度上的差异的,就比如做WB实验,经典的还是化学发光的方法,显色的方法只能得到信号比较强的蛋白,遇到信号比较弱的蛋白就误差很大了, 第二点,并不是所有能进核的蛋白都有结合DNA
science_sky 各位前辈,我接下来的实验要检测NFkB通路的活化状况,现在在订购抗体时遇到了问题:我准备订购IκB Antibody 和Phospho-IκB来反映NFkB通路的活化状况,发现Cell Signaling Technology提供了IκBα ,IκBβ ,IκBε和IκB-ζ和相应的磷酸化抗体,我应该订购那种亚型的抗体呢?希望前辈给于指点。 00501101 你看你要做什么检测IκB抑制的是NF
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