GFP antibody

Figure 1. Western blot of GFP recombinant protein detected with GeneTex polyclonal anti-GFP antibody. Lane 1 shows detection of a 33 kDa band corresponding to a GFP containing recombinant protein (arrowhead) expressed in HeLa cells. Lane 2 shows no staining of a mock transfected HeLa cell lysate. After blocking the membrane was probed with the primary antibody diluted to 1 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of donkey anti-Goat IgG [H&L].

Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus in Mus musculus (coronal view, 20X magnification). GeneTex polyclonal goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect eGFP. Fluorchrome conjugated anti-goat IgG secondary antibody was used for detection at 1:500. Sections were counterstained with DAPI. Personal Communication, Daniel A. Lee, Johns Hopkins School of Medicine, Baltimore, MD.

Figure 2. Immunofluorescence microscopy. GeneTex polyclonal anti-GFP antibody at a 1:1,000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system. Fluorochrome conjugated anti-Goat secondary antibody was used for detection at 1:300. Panel A shows a lateral view (ventral left) and Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). Personal Communication, Helmata Mistry, Washington University School of Medicine, St. Louis, MO.

Tissue: E5.5 Hex-GFP transgenic mouse embryo. Primary antibody: Goat anti-GFP was used at 1:500 dilution. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.