- ￥5600
- GeneTex
- 美国
- GTX24749
- 2025年07月14日
- WB, ICC/IF, IHC-P, FACS, Functional Assay, IHC, IHC (Free Floating)
- Rabbit
- Human, Mouse, Rat, Dog
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- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 199. The sequence is conserved in mouse and rat.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Dog
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX24749
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, FACS, Functional Assay, IHC, IHC (Free Floating)
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Tau (phospho Ser199)
- 抗体英文名：
Tau (phospho Ser199) antibody
- 抗体名：
Tau (phospho Ser199) 抗体
- 规格：
50 μl
IHC-P analysis of human brain tissue using GTX24749 Tau (phospho Ser199) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100
WB (peptide competition) analysis of human recombinant Tau untreated (Lane 1) or treated with GSK-3 beta (1 μg per μg Tau) for 45 minutes (Lanes 2-5) using GTX24749 Tau (phospho Ser199) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5)control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
IHC-P analysis of mouse brain tissue using GTX24749 Tau (phospho Ser199) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100
ICC/IF analysis of SH-SY5Y cells using GTX24749 Tau (phospho Ser199) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.25% Triton„ X-100 for 10 minutes
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文献和实验Zeng Y.Q. et al., Journal of Functional Foods 2021 77()
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