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Tau (phospho Se
r199) antibodyThe antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains serine 199. The sequence is conserved in mouse and rat.
IgG
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Polyclonal
Unconjugated
Human, Mouse, Rat, Dog
12 months from the shipping date of the product.
Human
GTX24749
Primary Antibodies
Available
GeneTex
Rabbit
WB, ICC/IF, IHC-P, FACS, Functional Assay, IHC, IHC (Free Floating)
Batch dependent (Please refer to the vial label for the specific concentration.)
Tau (phospho Ser199)
Tau (phospho Ser199) antibody
Tau (phospho Ser199) 抗体
50 μl
IHC-P analysis of human brain tissue using GTX24749 Tau (phospho Ser199) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100
WB (peptide competition) analysis of human recombinant Tau untreated (Lane 1) or treated with GSK-3 beta (1 μg per μg Tau) for 45 minutes (Lanes 2-5) using GTX24749 Tau (phospho Ser199) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5)control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
IHC-P analysis of mouse brain tissue using GTX24749 Tau (phospho Ser199) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100
ICC/IF analysis of SH-SY5Y cells using GTX24749 Tau (phospho Ser199) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.25% Triton„ X-100 for 10 minutes
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