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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 标记物:
HRP
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX225857-01
- 级别:
Research Kits
- 库存:
Available
- 供应商:
GeneTex
- 应用范围:
WB
- 浓度:
0.03 mg/ml (Please refer to the vial label for the specific concentration.)
- 抗体英文名:
EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G)
- 抗体名:
EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G)
- 规格:
250 μl/50 μl
| 规格: | 250 μl | 产品价格: | ¥4700.0 |
|---|---|---|---|
| 规格: | 50 μl | 产品价格: | ¥2000.0 |
EasyBlot anti-Mouse IgG Kit (Optimized for Prot A/G) (GTX225857-01) was used as a secondary reagent for immunoprecipitation analysis.
HepG2 lysates were immunoprecipitated using 5 ug of normal rabbit IgG or ELP3 antibody [GT11811] (GTX631395).
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文献和实验Lin TF et al., J Biol Chem 2014 (PMID:25008323)
Takeuchi Y et al., Medicines (Basel) 2020 (PMID:32384815)
Jean-Michel Paumier et al., FASEB 2018 (Epub)
Kubo Y et al., Front Microbiol 2017 (PMID:28900422)
the anti-Flag-M2 beads. Response: This has been changed accordingly and the saved input is now in step 14 of Part-II. 5.For preparation of genomic DNA by Qiagen DNeasy kit, guanidine hydrochloride would be used at the lysis step. Can DNA (or RNA)-siRNA
Chromosome Conformation Capture (3C) (PROT05)
-X to a final concentration of 1.8% (180µl of 20% Triton-X) to sequester the SDS. Incubate 1 hour at 37°C with shaking; Count the nuclei using a haemocytometer; Use a 1x106 nuclei aliquot (~15µg) for digestion. Digest overnight at 37°C
, even for a novice. The biggest advantage of the ChIP-IT kit is that it contains both positive (anti-TFIIB) and negative (negative IgG) control antibodies for the ChIP. This feature enables the user to determine if results are due to ChIP or the antibody
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