- ￥5300
- GeneTex
- 美国
- GTX22480
- 2025年07月16日
- WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to KLH - KKRPQRAT(pS)NVFAMFD (aa 12-27).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX22480
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody detects phosphorylated Ser19 Myosin Light Chain 2. The sequence around Ser19 is conserved among MYL9, MYL12A and MYL12B protein.
- 抗体英文名：
Myosin Light Chain 2 (phospho Ser19) antibody
- 抗体名：
Myosin Light Chain 2 (phospho Ser19) 抗体
- 规格：
50 μg
Western blot of Myosin Light chain (phospho Ser20) antibody (GTX22480). Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50 ng per lane. Primary antibody at 1:1,000 overnight at 4ºC. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 60 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 20 kDa.
Myosin Light chain (phospho Ser20) antibody (GTX22480) was used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system
Myosin Light chain (phospho Ser20) antibody (GTX22480) was used at 2.5 μg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
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文献和实验Carpenter AC et al., Development 2015 (PMID:25715397)
Plageman TF Jr et al., Development 2011 (PMID:22031541)
Chauhan BK et al., Proc Natl Acad Sci U S A 2011 (PMID:22021442)
Chauhan BK et al., Development 2009 (PMID:19820184)
Smith R et al., J Neurosci 2017 (PMID:28729440)
Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
构成肌球蛋白的低分子量（1.5— 2.5万）的亚单位。一分子肌球蛋白有 4个。肌球蛋白有在腺苷三磷酸酶（ ATP ase）作用中不可缺少的基本 L链和调节 ATP ase作用的调节 L链。从兔的白肌除去调节 L链（ L2 ：由 168个氨基酸所成）不影响肌球蛋白的作用。基本 L链（ L1 ∶ 190个氨基酸、 L3 ∶ 149个氨基酸）是必要的。兔白肌的肌球蛋白分子，根据 L链的不同而分为 3种，即每分子肌球蛋白中都含有两个 L2 ，其它两个则因种类不同而异，即分 L1
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