- ￥4300
- GeneTex
- 美国
- GTX18184
- 2025年07月15日
- WB, ICC/IF, IP, ELISA
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
HHHHHH (6x His) synthetic peptide conjugated to KLH
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX18184
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP, ELISA
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
- 抗体英文名：
6X His tag antibody [HIS.H8]
- 抗体名：
6X His tag 抗体 [HIS.H8]
- 规格：
100 μg
Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)
Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5μg/mL)
TOP: untransfected control; BOTTOM: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
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文献和实验Mabry R et al., MAbs 2010 (PMID:20065654)
Christine Kornelia Ott et al., 0 2015
Genotyping using Affymetrix arrays
is added once again and washed extensively with 6X SSPE and 0.01% Tween-20 at 30 °C. The SAPE and antibody were added to arrays in 6X SSPE, 1X Denhardt’s solution and 0.01% Tween-20 at 25 °C for 10 minutes each. Following the final wash, the arrays are kept
Geno typing using Affymetrix arrays
C. The SAPE and antibody were added to arrays in 6X SSPE, 1X Denhardt’s solution and 0.01% Tween-20 at 25 °C for 10 minutes each. Following the final wash, the arrays are kept in Holding buffer (100mM MES, 1M [Na ], 0.01% Tween-20). The washing
-20 at 30 ℃. The SAPE and antibody were added to arrays in 6X SSPE, 1X Denhardt’s solution and 0.01% Tween-20at 25 ℃ for 10 minutes each. Following the final wash, the arrays are kept in Holding buffer (100mM MES, 1M [Na+], 0.01% Tween-20). The washing
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