- ￥5300
- GeneTex
- 美国
- GTX18147
- 2025年07月14日
- ICC/IF, IHC-P, IHC
- Mouse
- Human, Mouse, Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
N-terminal decapeptide of the alpha smooth muscle isoform of Actin; acetylated at the N-terminus. Epitope: Acetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth Actin.
- 亚型：
IgG2a, Kappa
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rabbit
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX18147
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ICC/IF, IHC-P, IHC
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
alpha Smooth Muscle Actin
- 抗体英文名：
alpha Smooth Muscle Actin antibody [0.N.5]
- 抗体名：
alpha Smooth Muscle Actin 抗体 [0.N.5]
- 规格：
250 μl
IHC-P analysis of human leiomyoma tissue using GTX18147 alpha Smooth Muscle Actin antibody [0.N.5].
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文献和实验Otto C et al., Exp Ther Med 2012 (PMID:22969904)
Taghizadeh H et al., J Pers Med 2022 (PMID:36294740)
Xie X et al., Front Bioeng Biotechnol 2022 (PMID:36605251)
Chen C et al., Front Bioeng Biotechnol 2022 (PMID:36406232)
Yuhong Liu et al., Front Bioeng Biotechnol 2022 (PMID:35402413)
Riggle BA et al., J Clin Invest 2019 (PMID:31821175)
Klarquist J et al., Am J Pathol 2009 (PMID:19893037)
Labeling Muscle Actin with N-(1-pyrene)iodoacetamide
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0. 2.0.5 mM ATP,2 mM MgCl2,100 mM boric acid,pH 8.3 at 4℃,10 ml. 3.N-(1-pyrene)iodoacetamide (Molecular probes P-29,m.w.385) 4.50Ti tubes,small vial
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
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