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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
This monoclonal antibody was raised against a synthetic peptide containing amino acid residues 96-113 (RVELLKKEIDKFKDKKEV) of human kB-Ras1.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Bovine, Dog, Pig, Horse, Xenopus, Primate
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX13666
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB
- 浓度:
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
NKIRAS1
- 抗体英文名:
NKIRAS1 antibody [184C278]
- 抗体名:
NKIRAS1 抗体 [184C278]
- 规格:
100 μg
WB analysis of 293 cell lysate using GTX13666 NKIRAS1 antibody [184C278].
Dilution : 0.5 μg/ml
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文献和实验(1995) The 14-3-3 family of proteins plays a role in a wide variety of cellular functions including regulation of protein kinase C and exocytosis. Using antisera specific for the N termini of 14-3-3 isoforms described previously and an additional
ELISPOT (Enzyme-linked ImmunoSPOT)
capture antibody in sterile ELISPOT Coating Buffer, as noted on Certificate of Analysis which is included with the reagent set. Coat ELISPOT plate with 100µl/well of capture antibody solution. Incubate at 4°C overnight. Decant or aspirate coating
, Stuttgart, Germany) 。 ( 9 ) 成像板(BAS-SR 2025,Fujifilm,Japan) 。 ( 10 ) 磷屏显像仪(BAS-Reader-5000,Fujifilm) 。4. 注释 ( 1 ) 我们建议在激酶中加入甘油 [ 终浓度为 20% ( V/V)] 后保存在 -80°C,或将激酶在合适的缓冲液环境中冷冻干燥后保存在 -80°C,使用前,冻干的激酶要先用 ddH2O 溶解。 ( 2 ) 在进行基于芯片的激酶实验前,我们建议对激酶制备物进行胶内活性
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