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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the C-terminus region of human Rubicon. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX129096
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IP
- 浓度:
0.48 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Rubicon
- 抗体英文名:
Rubicon antibody
- 抗体名:
Rubicon 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rubicon antibody (GTX129096) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Rubicon antibody detects Rubicon protein by western blot analysis.
A. 30 ug 293T whole cell extract
B. 30 whole cell extract of human Rubicon-transfected 293T cells
5 % SDS-PAGE
Rubicon antibody (GTX129096) dilution: 1:20000
Rubicon antibody detects Rubicon protein by western blot analysis.
A. 30 μg MDA-MB-231 whole cell extract
5 % SDS-PAGE
Rubicon antibody (GTX129096) dilution: 1:1000
Rubicon antibody immunoprecipitates Rubicon protein in IP experiments.
IP samples: whole cell extract of Rubicon-transfected 293T cells
A. 30 μg 293T whole cell extract of Rubicon-protein expressing cell
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Rubicon protein by 4 μg Rubicon antibody (GTX129096)
5 % SDS-PAGE
The immunoprecipitated Rubicon protein was detected by Rubicon antibody (GTX129096) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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- 作者
- 内容
- 询问日期
文献和实验Quach C et al., Nat Commun 2019 (PMID:31831743)
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