- ￥7500
- GeneTex
- 美国
- GTX60828
- 2025年07月14日
- WB, ICC/IF, Dot, ELISA, ChIP assay, Protein Array
- Rabbit
- Human, Mouse
企业认证
相关产品推荐更多 >
![Aurora B antibody [AURKB/1521]](https://img1.dxycdn.com/2022/0328/328/8337496784089600453-14.jpg!wh200)
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The region of histone H4 containing the trimethylated lysine 20 (H4K20me3), using a KLH-conjugated synthetic peptide.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60828
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, Dot, ELISA, ChIP assay, Protein Array
- 浓度：
0.94 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H4K20me3 (trimethyl Lys20)
- 抗体英文名：
Histone H4K20me3 (trimethyl Lys20) antibody - ChIP grade
- 抗体名：
Histone H4K20me3 (trimethyl Lys20) 抗体 - ChIP grade
- 规格：
50 μg
Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H4K20 using GTX60828 Histone H4K20me3 (trimethyl Lys20) antibody - ChIP grade.
Dilution : 1:20,000
ICC/IF analysis of methanol fixed HeLa cells using GTX60828 Histone H4K20me3 (trimethyl Lys20) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500
Protein Array analysis of an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B using GTX60828 Histone H4K20me3 (trimethyl Lys20) antibody - ChIP grade.This figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.
Dilution : 1:10,000
WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60828 Histone H4K20me3 (trimethyl Lys20) antibody - ChIP grade.
Dilution : 1:1,000
ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60828 Histone H4K20me3 (trimethyl Lys20) antibody - ChIP grade. The chromatin was spiked with a panel of in vitro assembled nucleosomes, each containing a specific lysine methylation (SNAP-ChIP K-MetStat Panel, Epicypher). A titration consisting of 0.2, 0.5, 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Figure 1A. Quantitative PCR was performed with primers specific for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the ZNF10 gene and the Sat2 satellite repeat, used as positive controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H4K20me1, H4K20me2, H4K2me3 and the unmodified H4K20 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H4K20me3 modification.
ELISA analysis of peptide containing the histone modification of interest using GTX60828 Histone H4K20me3 (trimethyl Lys20) antibody - ChIP grade.
ChIP analysis of sheared chromatin from 1x10⁵ K562 cells using GTX60828 Histone H4K20me3 (trimethyl Lys20) antibody - ChIP grade. The IP'd DNA was analysed by QPCR as described above (figure 2A). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2B shows the signal distribution along the long arm of chromosome 19 and a zoomin to an enriched region containing several ZNF repeat genes. Figure 2C and D show the enrichment in the telomeric region of chromosome 12, also containing several ZNF repeat genes, and at ZNF510 on chromosome 9, respectively. The position of the amplicon used for ChIP-qPCR is indicated by an arrow.
Antibody amount : 0.5μg
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Chromatin Immunoprecipitation (ChIP)
of formaldehyde in the ChIP method was pioneered by Solomon and Varshavsky (Solomon et al. 1988). In their original studies, the association of histone H4 and RNA polymerase II with the Drosophila hsp70 genes was monitored. Cells were treated with formaldehyde
. However, to address in detail what happens at specific sites in vivo, chromatin immunoprecipitation (ChIP) is the method of choice. Here, we describe how ChIP can be performed on non-fixed chromatin from animal cells or tissues (fresh or frozen) to analyze histone
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
技术资料暂无技术资料 索取技术资料
