- ￥1700 - 4000
- GeneTex
- 美国
- GTX115045
- 2025年07月16日
- WB, IHC-Wm, IP, ELISA
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The immunogen used to generate this antibody corresponds to 6X His tag
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX115045
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-Wm, IP, ELISA
- 浓度：
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
6X His tag
- 抗体英文名：
6X His tag antibody
- 抗体名：
6X His tag 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
His-tagged protein was immunoprecipitated from transfected cells using 8 ug of 6X His tag antibody (GTX115045). The precipitated His-tagged protein was detected by GTX115045 diluted at 1:500, and used EasyBlot anti-rabbit IgG (GTX221666-01) as secondary reagent.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody (GTX115045) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
6X His tag antibody detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 2.5 μg/ml 6x His antibody (GTX115045). Bound Abs were further detected by using HRP-conjugated goat anti-rabbit IgG (GTX213110-01) with HRP substrates.
Sample
A: 10 μg of non-transformed E. coli lysate
B: 10 μg of non-transformed E. coli lysate, induced
C: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced
D: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced
12% SDS PAGE
GTX115045 diluated at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Pohlers S et al., MBio 2017 (PMID:28143980)
Liu W et al., Microbiologyopen 2018 (PMID:29314743)
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Huang SW et al., Virus Res. 2015 (PMID:26657880)
Tseng YY et al., J Virol 2015 (PMID:25694596)
Chang CC et al., Sci Rep 2018 (PMID:29955155)
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Han-Hsiang Chen et al., J Biol Chem 2022 (PMID:35452675)
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Ewelina ?ojewska et al., Plant Biotechnology Reports 2019 (Epub)
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Purification and Characterization of His-Tagged Antibody Fragments
, typically His6 , fused to the recombinant protein. In the case of antibody fragments that are composed of different chains, e.g., Fv and Fab, the site of attachment for the His6 tag has to be carefully chosen, and mild chromatographic conditions
Purification and Analysis of Strep-tagged Antibody-Fragments
The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity towards streptavidin. This peptide – or its improved version Strep-tag II – can be fused to recombinant antibody fragments and other proteins in various fashions
Protein Antigen Expression in Escherichia coli for Antibody Production
Escherichia coli is a frequently used expression system for the generation of protein encoded by genes from diverse kingdoms and, thus, it is well suited for the production of protein antigens for antibody generation. It is a system of choice
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