- ￥1700 - 4000
- GeneTex
- 美国
- GTX114455
- 2025年07月13日
- WB, IP, ChIP assay
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human TFIIF. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX114455
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IP, ChIP assay
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Knockdown/Knockout validation was supported by references (PMID:26990993)
- 抗体英文名：
TFIIF antibody
- 抗体名：
TFIIF 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX114455 diluted at 1:3000
Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX114455 diluted at 1:3000
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of TFIIF protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIF by 2.5 μg of TFIIF antibody (GTX114455) 7.5% SDS-PAGE The immunoprecipitated TFIIF protein was detected by TFIIF antibody (GTX114455) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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文献和实验Sundararaman B et al., Mol Cell 2016 (PMID:26990993)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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